Concepts to automate the theoretical design of effective antisense oligonucleotides
نویسندگان
چکیده
منابع مشابه
Concepts to automate the theoretical design of effective antisense oligonucleotides
Among the large number of possible antisense species against a given target RNA, only a small number shows effective suppression of the target gene in living cells. In the case of short-chain antisense oligonucleotides (asON) which usually comprise less than approximately 25 nucleotides, local structures of the target RNA seem to be of particular importance for the extent of gene suppression. E...
متن کاملTheoretical and experimental approaches to design effective antisense oligonucleotides.
Among the large number of possible antisense oligonucleotides (asODN) against a given target nucleic acid, only a small number of species seems to give rise to satisfactorily strong inhibition of target gene expression in living cells. Therefore much attention is paid to strategies that help to successfully design effective asODN. Here, selected experimental approaches and theoretical concepts ...
متن کاملAntisense oligonucleotides: basic concepts and mechanisms.
Conceptual simplicity, the possibility of rational design, relatively inexpensive cost, and developments in the sequencing of human genome have led to the use of short fragments of nucleic acid, commonly called oligonucleotides, either as therapeutic agents or as tools to study gene function. Furthermore, in the past decade, the development of antisense oligonucleotide technologies as therapeut...
متن کاملAntisense oligonucleotides: from design to therapeutic application.
1. An antisense oligonucleotide (ASO) is a short strand of deoxyribonucleotide analogue that hybridizes with the complementary mRNA in a sequence-specific manner via Watson-Crick base pairing. Formation of the ASO-mRNA heteroduplex either triggers RNase H activity, leading to mRNA degradation, induces translational arrest by steric hindrance of ribosomal activity, interferes with mRNA maturatio...
متن کاملAntisense oligonucleotides and the rational design of new antitumor drugs.
suming and resource-costly. For colon cancer, as for many other tumors, this goal is far from accomplished nowadays despite considerable effort. High costs should include, as a relevant issue, the high number of agents to be assessed and then discarded along the various stages of selection. To render this search more efficient, both official agencies and private ventures tend to rationalize thi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioinformatics
سال: 2001
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/17.11.1058